Immunogenicity difference between the SARS coronavirus and the bat SARS-like coronavirus spike (S) proteins.
Identifieur interne : 002A97 ( Main/Exploration ); précédent : 002A96; suivant : 002A98Immunogenicity difference between the SARS coronavirus and the bat SARS-like coronavirus spike (S) proteins.
Auteurs : Peng Zhou [République populaire de Chine] ; Zhenggang Han ; Lin-Fa Wang ; Zhengli ShiSource :
- Biochemical and biophysical research communications [ 1090-2104 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Chiroptera (virologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Humains, Lignée cellulaire, Protéines de l'enveloppe virale (immunologie), Souris, Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Vaccins antiviraux (immunologie), Virus du SRAS (immunologie).
- MESH :
English descriptors
- KwdEn :
- Animals, Cell Line, Chiroptera (virology), Humans, Membrane Glycoproteins (immunology), Mice, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , immunology : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Chiroptera, Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Humans, Mice, Spike Glycoprotein, Coronavirus.
Abstract
SARS-like coronavirus (SL-CoV) in bats have a similar genomic organization to the human SARS-CoV. Their cognate gene products are highly conserved with the exception of the N-terminal region of the S proteins, which have only 63-64% sequence identity. The N-terminal region of coronavirus S protein is responsible for virus-receptor interaction. In this study, the immunogenicity of the SL-CoV S protein (S(SL)) was studied and compared with that of SARS-CoV (S(SARS)). DNA immunization in mice with S(SL) elicited a high titer of antibodies against HIV-pseudotyped S(SL). The sera had low cross-reactivity, but no neutralization activity, for the HIV-pseudotyped S(SARS). Studies using wild bat sera revealed that it is highly likely that the immunodominant epitopes overlap with the major neutralizing sites of the SL-CoV S protein. These results demonstrated that SL-CoV and SARS-CoV shared only a limited number of immunogenic epitopes in their S proteins and the major neutralization epitopes are substantially different. This work provides useful information for future development of differential serologic diagnosis and vaccines for coronaviruses with different S protein sequences.
DOI: 10.1016/j.bbrc.2009.07.025
PubMed: 19595990
Affiliations:
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Le document en format XML
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sortKey="Shi, Zhengli" sort="Shi, Zhengli" uniqKey="Shi Z" first="Zhengli" last="Shi">Zhengli Shi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19595990</idno><idno type="pmid">19595990</idno><idno type="doi">10.1016/j.bbrc.2009.07.025</idno><idno type="wicri:Area/PubMed/Corpus">001863</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001863</idno><idno type="wicri:Area/PubMed/Curation">001863</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001863</idno><idno type="wicri:Area/PubMed/Checkpoint">001859</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001859</idno><idno type="wicri:Area/Ncbi/Merge">001F65</idno><idno type="wicri:Area/Ncbi/Curation">001F65</idno><idno type="wicri:Area/Ncbi/Checkpoint">001F65</idno><idno type="wicri:Area/Main/Merge">002B47</idno><idno 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sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name></author><author><name sortKey="Shi, Zhengli" sort="Shi, Zhengli" uniqKey="Shi Z" first="Zhengli" last="Shi">Zhengli Shi</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="eISSN">1090-2104</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chiroptera (virology)</term><term>Humans</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Chiroptera (virologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Lignée cellulaire</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Souris</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Vaccins antiviraux (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Humans</term><term>Mice</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Lignée cellulaire</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">SARS-like coronavirus (SL-CoV) in bats have a similar genomic organization to the human SARS-CoV. Their cognate gene products are highly conserved with the exception of the N-terminal region of the S proteins, which have only 63-64% sequence identity. The N-terminal region of coronavirus S protein is responsible for virus-receptor interaction. In this study, the immunogenicity of the SL-CoV S protein (S(SL)) was studied and compared with that of SARS-CoV (S(SARS)). DNA immunization in mice with S(SL) elicited a high titer of antibodies against HIV-pseudotyped S(SL). The sera had low cross-reactivity, but no neutralization activity, for the HIV-pseudotyped S(SARS). Studies using wild bat sera revealed that it is highly likely that the immunodominant epitopes overlap with the major neutralizing sites of the SL-CoV S protein. These results demonstrated that SL-CoV and SARS-CoV shared only a limited number of immunogenic epitopes in their S proteins and the major neutralization epitopes are substantially different. This work provides useful information for future development of differential serologic diagnosis and vaccines for coronaviruses with different S protein sequences.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement></list><tree><noCountry><name sortKey="Han, Zhenggang" sort="Han, Zhenggang" uniqKey="Han Z" first="Zhenggang" last="Han">Zhenggang Han</name><name sortKey="Shi, Zhengli" sort="Shi, Zhengli" uniqKey="Shi Z" first="Zhengli" last="Shi">Zhengli Shi</name><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name></noCountry><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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